Title

Abnormal fertility, acrosome formation, IFT20 expression and localization in conditional Gmap210 knockout mice

UMMS Affiliation

Program in Molecular Medicine

Publication Date

2020-01-01

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Cellular and Molecular Physiology | Developmental Biology

Abstract

GMAP210 (TRIP11) is a cis-Golgi network-associated protein and a Golgi membrane receptor for IFT20, an intraflagellar transport component essential for male fertility and spermiogenesis in mice. To investigate the role of GMAP210 in male fertility and spermatogenesis, floxed Gmap210 mice were bred with Stra8-iCre mice so that the Gmap210 gene is disrupted in spermatocytes and spermatids in this study. The Gmap210(flox/flox): Stra8-iCre mutant mice showed no gross abnormalities and survived to adulthood. In adult males, testis and body weights showed no difference between controls and mutant mice. Low-magnification histological examination of the testes revealed normal seminiferous tubule structure, but sperm counts and fertility were significantly reduced in mutant mice compared with controls. Higher resolution examination of the mutant seminiferous epithelium showed that nearly all sperm had more oblong, abnormally shaped heads, while the sperm tails appeared to have normal morphology. Electron microscopy also revealed abnormally shaped sperm heads but normal axoneme core structure; some sperm showed membrane defects in the midpiece. In mutant mice, expression levels of IFT20 and other selective acrosomal proteins were significantly reduced, and their localization was also affected. Peanut-lectin, an acrosome maker, was almost absent in the spermatids and epididymal sperm. Mitochondrion staining was highly concentrated in the heads of sperm, suggesting that the midpieces were coiling around or aggregating near the heads. Defects in acrosome biogenesis were further confirmed by electron microscopy. Collectively, our findings suggest that GMAP210 is essential for acrosome biogenesis, normal mitochondrial sheath formation, and male fertility, and it determines expression levels and acrosomal localization of IFT20 and other acrosomal proteins.

Keywords

GMAP210, IFT20, Trip11, acrosome formation, male fertility

DOI of Published Version

10.1152/ajpcell.00517.2018

Source

Wang Z, Shi Y, Ma S, Huang Q, Yap YT, Shi L, Zhang S, Zhou T, Li W, Hu B, Zhang L, Krawetz SA, Pazour GJ, Hess RA, Zhang Z. Abnormal fertility, acrosome formation, IFT20 expression and localization in conditional Gmap210 knockout mice. Am J Physiol Cell Physiol. 2020 Jan 1;318(1):C174-C190. doi: 10.1152/ajpcell.00517.2018. Epub 2019 Oct 2. PMID: 31577511; PMCID: PMC6985835. Link to article on publisher's site

Journal/Book/Conference Title

American journal of physiology. Cell physiology

Related Resources

Link to Article in PubMed

PubMed ID

31577511

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