Comprehensive mapping of long-range interactions reveals folding principles of the human genome
Authors
Lieberman-Aiden, Erezvan Berkum, Nynke L.
Williams, Louise
Imakaev, Maxim
Ragoczy, Tobias
Telling, Agnes
Amit, Ido
Lajoie, Bryan R.
Sabo, Peter J.
Dorschner, Michael O.
Sandstrom, Richard
Berstein, Bradley
Bender, M. A.
Groudine, Mark
Gnirke, Andreas
Stamatoyannopoulos, John A.
Mirny, Leonid A.
Lander, Eric S.
Dekker, Job
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Gene Function and Expression
Document Type
Journal ArticlePublication Date
2009-10-10Keywords
BiotinCell Line, Transformed
Cell Nucleus
Chromatin
Chromatin Immunoprecipitation
*Chromosomes, Human
Computational Biology
DNA
Gene Library
*Genome, Human
Humans
In Situ Hybridization, Fluorescence
Models, Molecular
Monte Carlo Method
Nucleic Acid Conformation
Principal Component Analysis
Protein Conformation
Sequence Analysis, DNA
Genetics and Genomics
Metadata
Show full item recordAbstract
We describe Hi-C, a method that probes the three-dimensional architecture of whole genomes by coupling proximity-based ligation with massively parallel sequencing. We constructed spatial proximity maps of the human genome with Hi-C at a resolution of 1 megabase. These maps confirm the presence of chromosome territories and the spatial proximity of small, gene-rich chromosomes. We identified an additional level of genome organization that is characterized by the spatial segregation of open and closed chromatin to form two genome-wide compartments. At the megabase scale, the chromatin conformation is consistent with a fractal globule, a knot-free, polymer conformation that enables maximally dense packing while preserving the ability to easily fold and unfold any genomic locus. The fractal globule is distinct from the more commonly used globular equilibrium model. Our results demonstrate the power of Hi-C to map the dynamic conformations of whole genomes.Source
Science. 2009 Oct 9;326(5950):289-93. Link to article on publisher's siteDOI
10.1126/science.1181369Permanent Link to this Item
http://hdl.handle.net/20.500.14038/44094PubMed ID
19815776Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1126/science.1181369