Document Type
Book ChapterPublication Date
2013-09-10Keywords
AdipocytesAnimals
Animals, Genetically Modified
Caenorhabditis elegans
Humans
Longevity
Oxidative Stress
Sirtuins
Biochemistry
Computational Biology
Genetics and Genomics
Molecular Biology
Metadata
Show full item recordAbstract
The nematode Caenorhabditis elegans (C. elegans) has four Sir2 paralogs, sir-2.1, sir-2.2, sir-2.3, and sir-2.4. Thus far, most of the research tools to study worm sirtuins have been developed for sir-2.1, due to its homology to yeast SIR2 and human SIRT1. Here, we have compiled a listing of the currently available strains (including both loss-of-function alleles and transgenics), antibodies, and RNAi constructs relevant to studies on all C. elegans sirtuin family members. We also describe the methods used in the analysis of C. elegans sirtuin function, including life span analysis, various stress-resistance assays, and fat content analysis and provide an overview of all phenotypic data relevant to C. elegans sir-2.1.Source
Viswanathan M, Tissenbaum HA. C. elegans sirtuins. Methods Mol Biol. 2013;1077:39-56. doi: 10.1007/978-1-62703-637-5_3. Link to article on publisher's siteDOI
10.1007/978-1-62703-637-5_3Permanent Link to this Item
http://hdl.handle.net/20.500.14038/44029PubMed ID
24014398Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1007/978-1-62703-637-5_3