C. elegans sirtuins

UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine



Document Type

Book Chapter

Medical Subject Headings

Adipocytes; Animals; Animals, Genetically Modified; Caenorhabditis elegans; Humans; Longevity; Oxidative Stress; Sirtuins


Biochemistry | Computational Biology | Genetics and Genomics | Molecular Biology


The nematode Caenorhabditis elegans (C. elegans) has four Sir2 paralogs, sir-2.1, sir-2.2, sir-2.3, and sir-2.4. Thus far, most of the research tools to study worm sirtuins have been developed for sir-2.1, due to its homology to yeast SIR2 and human SIRT1. Here, we have compiled a listing of the currently available strains (including both loss-of-function alleles and transgenics), antibodies, and RNAi constructs relevant to studies on all C. elegans sirtuin family members. We also describe the methods used in the analysis of C. elegans sirtuin function, including life span analysis, various stress-resistance assays, and fat content analysis and provide an overview of all phenotypic data relevant to C. elegans sir-2.1.

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Citation: Viswanathan M, Tissenbaum HA. C. elegans sirtuins. Methods Mol Biol. 2013;1077:39-56. doi: 10.1007/978-1-62703-637-5_3. Link to article on publisher's site

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