An optimized two-finger archive for ZFN-mediated gene targeting
Program in Gene Function and Expression; Department of Biochemistry and Molecular Pharmacology
Zinc Fingers; Gene Targeting
Genetics and Genomics
The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
DOI of Published Version
Nat Methods. 2012 Apr 29. doi: 10.1038/nmeth.1994. [Epub ahead of print]
Gupta A, Rayla AL, Lakshmanan A, Wolfe SA, Christensen RG, Stormo GD. (2012). An optimized two-finger archive for ZFN-mediated gene targeting. Program in Gene Function and Expression Publications. https://doi.org/10.1038/nmeth.1994. Retrieved from https://escholarship.umassmed.edu/pgfe_pp/186