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Authors
Walhout, Albertha J. M.Document Type
Journal ArticlePublication Date
2011-11-29Keywords
Two-Hybrid System TechniquesHigh-Throughput Screening Assays
Gene Regulatory Networks
Protein Interaction Mapping
Transcription Factors
Caenorhabditis elegans Proteins
Genetics and Genomics
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Show full item recordAbstract
The Caenorhabditis elegans hermaphrodite is a complex multicellular animal that is composed of 959 somatic cells. The C. elegans genome contains approximately 20,000 protein-coding genes, 940 of which encode regulatory transcription factors (TFs). In addition, the worm genome encodes more than 100 microRNAs and many other regulatory RNA and protein molecules. Most C. elegans genes are subject to regulatory control, most likely by multiple regulators, and combined, this dictates the activation or repression of the gene and corresponding protein in the relevant cells and under the appropriate conditions. A major goal in C. elegans research is to determine the spatiotemporal expression pattern of each gene throughout development and in response to different signals, and to determine how this expression pattern is accomplished. Gene regulatory networks describe physical and/or functional interactions between genes and their regulators that result in specific spatiotemporal gene expression. Such regulators can act at transcriptional or post-transcriptional levels. Here, I will discuss the methods that can be used to delineate gene regulatory networks in C. elegans. I will mostly focus on gene-centered yeast one-hybrid (Y1H) assays that are used to map interactions between non-coding genic regions, such as promoters, and regulatory TFs. The approaches discussed here are not only relevant to C. elegans biology, but can also be applied to other model organisms and humans.Source
Methods Cell Biol. 2011;106:271-88. Link to article on publisher's siteDOI
10.1016/B978-0-12-544172-8.00010-4Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43964PubMed ID
22118281Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/B978-0-12-544172-8.00010-4