Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish
Program in Gene Function and Expression; Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Zebrafish; Zebrafish Proteins; Zinc Fingers; Gene Silencing; GATA2 Transcription Factor
Genetics and Genomics
Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. However, construction of target-specific ZFNs is technically challenging. Here, we evaluate a straightforward modular assembly-based approach for ZFN construction and gene inactivation in zebrafish. From an archive of 27 different zinc-finger modules, we assembled more than 70 different zinc-finger cassettes and evaluated their specificity using a bacterial one-hybrid assay. In parallel, we constructed ZFNs from these cassettes and tested their ability to induce lesions in zebrafish embryos. We found that the majority of zinc-finger proteins assembled from these modules have favorable specificities and nearly one-third of modular ZFNs generated lesions at their targets in the zebrafish genome. To facilitate the application of ZFNs within the zebrafish community we constructed a public database of sites in the zebrafish genome that can be targeted using this archive. Importantly, we generated new germline mutations in eight different genes, confirming that this is a viable platform for heritable gene inactivation in vertebrates. Characterization of one of these mutants, gata2a, revealed an unexpected role for this transcription factor in vascular development. This work provides a resource to allow targeted germline gene inactivation in zebrafish and highlights the benefit of a definitive reverse genetic strategy to reveal gene function.
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Citation: Development. 2011 Oct;138(20):4555-64. Link to article on publisher's site
Zhu, Cong; Smith, Tom; McNulty, Joseph C.; Rayla, Amy L.; Lakshmanan, Abirami; Siekmann, Arndt F.; Buffardi, Matthew; Meng, Xiangdong; Shin, Jimann; Padmanabhan, Arun; Cifuentes, Daniel; Giraldez, Antonio J.; Look, A. Thomas; Epstein, Jonathan A.; Lawson, Nathan D.; and Wolfe, Scot A., "Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish" (2011). Program in Gene Function and Expression Publications and Presentations. 173.