The three-dimensional architecture of a bacterial genome and its alteration by genetic perturbation
Program in Gene Function and Expression; Department of Biochemistry and Molecular Pharmacology; Program in Systems Biology
Caulobacter crescentus; Genome, Bacterial; Chromosomes, Bacterial; Imaging, Three-Dimensional
Genetics and Genomics
We have determined the three-dimensional (3D) architecture of the Caulobacter crescentus genome by combining genome-wide chromatin interaction detection, live-cell imaging, and computational modeling. Using chromosome conformation capture carbon copy (5C), we derive approximately 13 kb resolution 3D models of the Caulobacter genome. The resulting models illustrate that the genome is ellipsoidal with periodically arranged arms. The parS sites, a pair of short contiguous sequence elements known to be involved in chromosome segregation, are positioned at one pole, where they anchor the chromosome to the cell and contribute to the formation of a compact chromatin conformation. Repositioning these elements resulted in rotations of the chromosome that changed the subcellular positions of most genes. Such rotations did not lead to large-scale changes in gene expression, indicating that genome folding does not strongly affect gene regulation. Collectively, our data suggest that genome folding is globally dictated by the parS sites and chromosome segregation.
DOI of Published Version
Mol Cell. 2011 Oct 21;44(2):252-64. Link to article on publisher's site
Umbarger, Mark A.; Toro, Esteban; Wright, Matthew A.; Porreca, Gregory J.; Bau, Davide; Hong, Sun-Hae; Fero, Michael J.; Zhu, Lihua Julie; Marti-Renom, Marc A.; McAdams, Harley H.; Shapiro, Lucy; Dekker, Job; and Church, George M., "The three-dimensional architecture of a bacterial genome and its alteration by genetic perturbation" (2011). Program in Gene Function and Expression Publications and Presentations. 171.