A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome
Program in Gene Function and Expression; Program in Molecular Medicine
Animals; Caenorhabditis elegans; Databases as Topic; Models, Molecular; Open Reading Frames; Peptide Hydrolases; Plasmids; Polymerase Chain Reaction; *Proteasome Endopeptidase Complex; Protein Binding; Transformation, Genetic; Two-Hybrid System Techniques
Genetics and Genomics
The ubiquitin-proteasome proteolytic pathway is pivotal in most biological processes. Despite a great level of information available for the eukaryotic 26S proteasome-the protease responsible for the degradation of ubiquitylated proteins-several structural and functional questions remain unanswered. To gain more insight into the assembly and function of the metazoan 26S proteasome, a two-hybrid-based protein interaction map was generated using 30 Caenorhabditis elegans proteasome subunits. The results recapitulate interactions reported for other organisms and reveal new potential interactions both within the 19S regulatory complex and between the 19S and 20S subcomplexes. Moreover, novel potential proteasome interactors were identified, including an E3 ubiquitin ligase, transcription factors, chaperone proteins and other proteins not yet functionally annotated. By providing a wealth of novel biological hypotheses, this interaction map constitutes a framework for further analysis of the ubiquitin-proteasome pathway in a multicellular organism amenable to both classical genetics and functional genomics.
DOI of Published Version
EMBO Rep. 2001 Sep;2(9):821-8. Link to article on publisher's site
Davy, Anne; Bello, Paul; Thierry-Mieg, Nicolas; Vaglio, Philippe; Hitti, Joseph; Doucette-Stamm, Lynn; Thierry-Mieg, Danielle; Reboul, Jerome; Boulton, Simon J.; Walhout, Albertha J. M.; Coux, Olivier; and Vidal, Marc, "A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome" (2001). Program in Gene Function and Expression Publications and Presentations. 14.