Title

High-throughput yeast two-hybrid assays for large-scale protein interaction mapping

UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine

Publication Date

2001-06-14

Document Type

Article

Subjects

Culture Media; DNA, Recombinant; Fungal Proteins; Gene Library; Genes, Reporter; Polymerase Chain Reaction; Proteins; Saccharomyces cerevisiae; Transformation, Genetic; *Two-Hybrid System Techniques

Disciplines

Genetics and Genomics

Abstract

Protein-protein interactions play fundamental roles in many biological processes. Hence, protein interaction mapping is becoming a well-established functional genomics approach to generate functional annotations for predicted proteins that so far have remained uncharacterized. The yeast two-hybrid system is currently one of the most standardized protein interaction mapping techniques. Here, we describe the protocols for a semiautomated, high-throughput, Gal4-based yeast two-hybrid system.

DOI of Published Version

10.1006/meth.2001.1190

Source

Methods. 2001 Jul;24(3):297-306. Link to article on publisher's site

Journal/Book/Conference Title

Methods (San Diego, Calif.)

Comments

At the time of publication, Albertha J. Marian Walhout was not yet affiliated with the University of Massachusetts Medical School.

Related Resources

Link to Article in PubMed

PubMed ID

11403578

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