High-throughput yeast two-hybrid assays for large-scale protein interaction mapping
Program in Gene Function and Expression; Program in Molecular Medicine
Culture Media; DNA, Recombinant; Fungal Proteins; Gene Library; Genes, Reporter; Polymerase Chain Reaction; Proteins; Saccharomyces cerevisiae; Transformation, Genetic; *Two-Hybrid System Techniques
Genetics and Genomics
Protein-protein interactions play fundamental roles in many biological processes. Hence, protein interaction mapping is becoming a well-established functional genomics approach to generate functional annotations for predicted proteins that so far have remained uncharacterized. The yeast two-hybrid system is currently one of the most standardized protein interaction mapping techniques. Here, we describe the protocols for a semiautomated, high-throughput, Gal4-based yeast two-hybrid system.
DOI of Published Version
Methods. 2001 Jul;24(3):297-306. Link to article on publisher's site
Methods (San Diego, Calif.)
Walhout, Albertha J. M. and Vidal, Marc, "High-throughput yeast two-hybrid assays for large-scale protein interaction mapping" (2001). Program in Gene Function and Expression Publications and Presentations. 13.