Protein interaction mapping in C. elegans using proteins involved in vulval development

UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine



Document Type


Medical Subject Headings

Animals; Caenorhabditis elegans; *Caenorhabditis elegans Proteins; Cloning, Molecular; Databases, Factual; Female; Genes, Helminth; Genetic Vectors; *Genome; Helminth Proteins; Mutation; Open Reading Frames; Phenotype; Repressor Proteins; Retinoblastoma Protein; *Two-Hybrid System Techniques; Vulva


Genetics and Genomics


Protein interaction mapping using large-scale two-hybrid analysis has been proposed as a way to functionally annotate large numbers of uncharacterized proteins predicted by complete genome sequences. This approach was examined in Caenorhabditis elegans, starting with 27 proteins involved in vulval development. The resulting map reveals both known and new potential interactions and provides a functional annotation for approximately 100 uncharacterized gene products. A protein interaction mapping project is now feasible for C. elegans on a genome-wide scale and should contribute to the understanding of molecular mechanisms in this organism and in human diseases.

Rights and Permissions

Citation: Science. 2000 Jan 7;287(5450):116-22. Link to article on publisher's site


At the time of publication, Albertha J. Marian Walhout was not yet affiliated with the University of Massachusetts Medical School.

Related Resources

Link to Article in PubMed

PubMed ID