UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine

Publication Date

2006-02-13

Document Type

Book Chapter

Subjects

Caenorhabditis elegans; Caenorhabditis elegans Proteins; Protein Interaction Mapping; Two-Hybrid System Techniques; Tandem Mass Spectrometry

Disciplines

Genetics and Genomics

Abstract

Several forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).

DOI of Published Version

110.1895/wormbook.1.86.1

Source

Walhout, A.J.M. and Boulton, S.J. Biochemistry and molecular biology (February 13, 2006), WormBook, ed. The C. elegans Research Community, WormBook, http://dx.doi.org/110.1895/wormbook.1.86.1, http://www.wormbook.org.

Journal/Book/Conference Title

The C. elegans Research Community, WormBook

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