UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine

Publication Date


Document Type

Book Chapter


Caenorhabditis elegans; Caenorhabditis elegans Proteins; Protein Interaction Mapping; Two-Hybrid System Techniques; Tandem Mass Spectrometry


Genetics and Genomics


Several forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).

DOI of Published Version



Walhout, A.J.M. and Boulton, S.J. Biochemistry and molecular biology (February 13, 2006), WormBook, ed. The C. elegans Research Community, WormBook,,

Journal/Book/Conference Title

The C. elegans Research Community, WormBook



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