Engineered telomeres in transgenic Xenopus laevis
Department of Cell and Developmental Biology
Medical Subject Headings
Animals; Animals, Genetically Modified; DNA; *Epigenesis, Genetic; Genetic Engineering; In Situ Hybridization, Fluorescence; Models, Animal; Telomere; Xenopus laevis
Cell Biology | Developmental Biology | Molecular Biology | Molecular Genetics
The expanding roles of telomeres in epigenetic gene regulation, nuclear organization, and human disease have necessitated the establishment of model organisms in which to study telomere function under normal developmental conditions. We present an efficient system for generating numerous vertebrate animals containing engineered telomeres using a Xenopus laevis transgenesis technique. Our results indicate Xenopus zygotes efficiently recognize telomeric repeats at chromosome break points and form telomeric complexes thus generating a new telomere. The resulting transgenic animals progress through normal development and successfully metamorphose into froglets despite the chromosome breakage. Overall, this presents an efficient mechanism for generating engineered telomeres in a vertebrate system and provides an opportunity to investigate epigenetic aspects of telomere function during normal vertebrate development.
Rights and Permissions
Citation: Transgenic Res. 2007 Jun;16(3):377-84.doi: 10.1007/s11248-007-9076-0. Link to article on publisher's site
Telomere, Transgenic Xenopus, laevis
Wuebbles, Ryan D. and Jones, Peter L., "Engineered telomeres in transgenic Xenopus laevis" (2007). Peter Jones Lab Publications. 8.