Title
Gene transfer in the lung using recombinant adeno-associated virus
UMMS Affiliation
Gene Therapy Center; Department of Pediatrics; Department of Microbiology and Physiological Systems
Publication Date
2012-08-01
Document Type
Book Chapter
Subjects
Dependovirus; Genetic Vectors; Gene Therapy; Transgenes; Gene Transfer Techniques; Lung
Disciplines
Allergy and Immunology | Genetics and Genomics | Medical Genetics | Pediatrics | Respiratory Tract Diseases
Abstract
Adeno-associated virus (AAV) is a small replication-deficient DNA virus belonging to the Parvovirinae family. It has a single-stranded ∼4.7-kb genome. Recombinant AAV (rAAV) is created by replacing the viral rep and cap genes with the transgene of interest along with promoter and polyadenylation sequences. The short viral inverted terminal repeats must remain intact for replication and packaging in production, as well as vector genome processing and persistence in the transduction process. The AAV capsid (serotype) determines the tissue tropism of the rAAV vector. In this unit we will discuss serotype selection for lung targeting along with the factors effecting efficient delivery of rAAV vectors to the murine lung. Detailed procedures for lung delivery (intranasal, orotracheal, and surgical tracheal injection), sample collection, and post-mortem tissue processing will be described. Curr. Protoc. Microbiol. 26:14D.2.1-14D.2.17. © 2012 by John Wiley & Sons, Inc.
DOI of Published Version
10.1002/9780471729259.mc14d02s26
Source
Curr Protoc Microbiol. 2012 Aug;Chapter 14:Unit14D.2. DOI: 10.1002/9780471729259.mc14d02s26
Journal/Book/Conference Title
Current Protocols in Microbiology
Related Resources
PubMed ID
22875566
Repository Citation
Gruntman, Alisha; Mueller, Christian; Flotte, Terence R.; and Gao, Guangping, "Gene transfer in the lung using recombinant adeno-associated virus" (2012). Pulmonary and Allergy. 71.
https://escholarship.umassmed.edu/peds_pulmonary/71