Frontline Science: Splenic progenitors aid in maintaining high neutrophil numbers at sites of sterile chronic inflammation
Department of Pediatrics, Division of Hematology Oncology; Program in Molecular Medicine, Diabetes Center of Excellence
Cell Biology | Hematology
Neutrophils are constantly generated from hematopoietic stem and progenitor cells in the bone marrow to maintain high numbers in circulation. A considerable number of neutrophils and their progenitors have been shown to be present in the spleen too; however, their exact role in this organ remains unclear. Herein, we sought to study the function of splenic neutrophils and their progenitors using a mouse model for sterile, peritoneal inflammation. In this microcapsule device implantation model, we show chronic neutrophil presence at implant sites, with recruitment from circulation as the primary mechanism for their prevalence in the peritoneal exudate. Furthermore, we demonstrate that progenitor populations in the spleen play a key role in maintaining elevated neutrophil numbers. Our results provide new insight into the role for splenic neutrophils and their progenitors and establish a model to study neutrophil function during sterile inflammation.
DOI of Published Version
J Leukoc Biol. 2016 Aug;100(2):253-60. doi: 10.1189/jlb.1HI0615-248RR. Epub 2016 Mar 10. Link to article on publisher's site
Journal of leukocyte biology
Jhunjhunwala, Siddharth; Alvarez, David; Aresta-DaSilva, Stephanie; Tang, Katherine; Tang, Benjamin C.; Greiner, Dale; Newburger, Peter E.; von Andrian, Ulrich H.; Langer, Robert; and Anderson, Daniel G., "Frontline Science: Splenic progenitors aid in maintaining high neutrophil numbers at sites of sterile chronic inflammation" (2016). Pediatric Publications and Presentations. 80.