Title
Lack of telomere shortening with age in mouse resting zone chondrocytes
UMMS Affiliation
Department of Pediatrics, Division of Endocrinology
Publication Date
2005-03-24
Document Type
Article
Subjects
Aging; Animals; Blotting, Southern; Cell Division; Chondrocytes; Growth Plate; Mice; Telomere
Disciplines
Pediatrics
Abstract
BACKGROUND AND AIM: Telomeres are hexameric repeat sequences that flank eukaryotic chromosomes. The telomere hypothesis of cellular aging proposes that replication of normal somatic cells leads to progressive telomere shortening which induces replicative senescence. Previous studies suggest that growth plate chondrocytes have a finite proliferative capacity in vivo. We therefore hypothesized that telomere shortening in resting zone chondrocytes leads to replicative senescence.
METHOD: To test this hypothesis we compared the telomere restriction fragment (TRF) length of Mus casteneus at 1, 4, 8, and 56 weeks of age.
RESULTS AND CONCLUSIONS: We found that TRF length did not diminish measurably with age, suggesting that telomere shortening in resting zone chondrocytes is not the mechanism that limits proliferation of growth plate chondrocytes in vivo.
DOI of Published Version
10.1159/000084687
Source
Horm Res. 2005;63(3):125-8. Epub 2005 Mar 24. Link to article on publisher's site
Journal/Book/Conference Title
Hormone research
Related Resources
PubMed ID
15795509
Repository Citation
Nwosu, Benjamin U.; Nilsson, Ola; Mitchum, Robert D. Jr.; Coco, Marilena; Barnes, Kevin M.; and Baron, Jeffrey, "Lack of telomere shortening with age in mouse resting zone chondrocytes" (2005). Pediatric Publications and Presentations. 3.
https://escholarship.umassmed.edu/peds_pp/3