Increased Toll-like receptor (TLR) mRNA expression in monocytes is a feature of metabolic syndrome in adolescents
UMass Chan Affiliations
Department of PediatricsDocument Type
Journal ArticlePublication Date
2013-02-01Keywords
Metabolic Syndrome XAdolescent
Immunity, Innate
Toll-Like Receptors
UMCCTS funding
Immunity
Nutritional and Metabolic Diseases
Pediatrics
Metadata
Show full item recordAbstract
BACKGROUND: Metabolic syndrome (MetSyn) is diagnosed frequently in some but not all overweight adolescents. Chronic inflammation, as seen in obesity, is strongly associated with MetSyn. OBJECTIVES: The aim of this pilot study was to assess the correlation between activation of the innate immune system and MetSyn, independent of body mass index (BMI), in a young population. METHODS: We quantitatively measured both systemic pro-inflammatory cytokines and gene expression of Toll-like receptors (TLRs) and downstream cytokines in circulating monocytes obtained from nine adolescents with metabolic syndrome (Overwt-MetSyn) and eight BMI-matched controls (Overwt-Healthy). RESULTS: The Overwt-MetSyn group demonstrated a significant elevation in expression of TLR2, TLR4, tumour necrosis factor-a (TNF a) and interleukin-6 (IL6) in peripheral monocytes, and increased circulating levels of TNF a and IL6 when compared with the Overwt-Healthy group. TLR2 (r = 0.78, P < 0.001), TLR4 (r = 0.57, P < 0.01) and TNF a (r = 0.61, P < 0.01) gene expression positively correlated with serum levels of TNF a. CONCLUSIONS: Our study suggests that activation of the innate immune pathway via TLRs may be partially responsible for the increased systemic inflammation seen in adolescents with MetSyn. the Study of Obesity.Source
Hardy, O. T., Kim, A., Ciccarelli, C., Hayman, L. L. and Wiecha, J. (2013), Increased Toll-like receptor (TLR) mRNA expression in monocytes is a feature of metabolic syndrome in adolescents. Pediatric Obesity, 8: e19–e23. doi: 10.1111/j.2047-6310.2012.00098.x Link to article on publisher's site
DOI
10.1111/j.2047-6310.2012.00098.xPermanent Link to this Item
http://hdl.handle.net/20.500.14038/43663PubMed ID
22991262Related Resources
ae974a485f413a2113503eed53cd6c53
10.1111/j.2047-6310.2012.00098.x