Design, Cloning, and In Vitro Screening of Artificial miRNAs to Silence Alpha-1 Antitrypsin
UMass Chan Affiliations
Department of Pediatrics, Division of Pulmonary and AllergyHorae Gene Therapy Center
Document Type
Book ChapterPublication Date
2017-07-28Keywords
AATAlpha-1 antitrypsin
RNA interference
RNAi
Short hairpin RNA; Z-AAT
miRNA
microRNA
shRNA
Genetics and Genomics
Molecular Biology
Research Methods in Life Sciences
Metadata
Show full item recordAbstract
This protocol describes the design, cloning, and in vitro screening of artificial microRNAs (miRNAs) to silence alpha-1 antitrypsin (AAT). This method would be of interest to silence AAT in a variety of in vitro or in vivo models, and prevalidated sequences against human AAT are provided. This simple 5-day protocol may more generally be used to design artificial miRNAs against any transcript.Source
Methods Mol Biol. 2017;1639:107-114. doi: 10.1007/978-1-4939-7163-3_10. Link to article on publisher's siteDOI
10.1007/978-1-4939-7163-3_10Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43593PubMed ID
28752450Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1007/978-1-4939-7163-3_10