UMass Chan Affiliations
Department of Pediatrics, Division of Pulmonary and AllergyHorae Gene Therapy Center
Document Type
Book ChapterPublication Date
2017-07-28Keywords
AATAlpha-1 antitrypsin
Direct ELISA
Enzyme-linked immunosorbent assay
Murine AAT
Molecular Biology
Research Methods in Life Sciences
Metadata
Show full item recordAbstract
This methods chapter elaborates on how a direct enzyme-linked immunosorbent assay (ELISA) is used to specifically detect and quantify murine alpha-1 antitrypsin (AAT). As a direct ELISA, it lacks some sensitivity as compared to the "sandwich" ELISA method; however, it does reliably differentiate between samples with varying amounts of the mouse AAT protein. This protocol relies on the principle of adsorption to coat each well with sera proteins, whereas detection occurs specifically using a two-step antibody combination. This procedure effectively identifies and quantifies murine AAT from a wide variety of samples including mouse serum, cell culture medium, and cell or tissue lysate.Source
Methods Mol Biol. 2017;1639:217-222. doi: 10.1007/978-1-4939-7163-3_21. Link to article on publisher's siteDOI
10.1007/978-1-4939-7163-3_21Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43591PubMed ID
28752461Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1007/978-1-4939-7163-3_21