Quantification of Z-AAT by a Z-Specific "Sandwich" ELISA
Horae Gene Therapy Center; Department of Pediatrics, Division of Pulmonary and Allergy
Molecular Biology | Research Methods in Life Sciences
This protocol describes an enzyme-linked immunosorbent assay (ELISA) to specifically detect Z-alpha-1 antitrypsin (AAT), the most common protein variant associated with alpha-1 antitrypsin deficiency. This "sandwich" ELISA relies on an anti-Z-AAT specific capture antibody and a HRP-conjugated anti-AAT detection antibody. This method would be of interest to identify and quantify Z-AAT in a variety of samples such as cell culture medium, cell or tissue lysate, animal or patient serum. Because this method is specific and sensitive, it would be particularly valuable for detection of Z-AAT in the presence of background M-AAT, for instance when quantifying silencing of Z-AAT in patients undergoing M-AAT augmentation therapy.
AAT, Alpha-1 antitrypsin, Enzyme-linked immunosorbent assay, Sandwich ELISA, UMMS-PiZ-mAb, Z-AAT
DOI of Published Version
Methods Mol Biol. 2017;1639:223-226. doi: 10.1007/978-1-4939-7163-3_22. Link to article on publisher's site
Methods in molecular biology (Clifton, N.J.)
Borel, Florie; Tang, Qiushi; and Mueller, Christian, "Quantification of Z-AAT by a Z-Specific "Sandwich" ELISA" (2017). Pediatric Publications and Presentations. 160.