UMass Chan Affiliations
Department of Pediatrics, Division of Pulmonary and AllergyHorae Gene Therapy Center
Document Type
Book ChapterPublication Date
2017-07-28Keywords
AATAlpha-1 antitrypsin
Enzyme-linked immunosorbent assay
Sandwich ELISA
UMMS-PiZ-mAb
Z-AAT
Molecular Biology
Research Methods in Life Sciences
Metadata
Show full item recordAbstract
This protocol describes an enzyme-linked immunosorbent assay (ELISA) to specifically detect Z-alpha-1 antitrypsin (AAT), the most common protein variant associated with alpha-1 antitrypsin deficiency. This "sandwich" ELISA relies on an anti-Z-AAT specific capture antibody and a HRP-conjugated anti-AAT detection antibody. This method would be of interest to identify and quantify Z-AAT in a variety of samples such as cell culture medium, cell or tissue lysate, animal or patient serum. Because this method is specific and sensitive, it would be particularly valuable for detection of Z-AAT in the presence of background M-AAT, for instance when quantifying silencing of Z-AAT in patients undergoing M-AAT augmentation therapy.Source
Methods Mol Biol. 2017;1639:223-226. doi: 10.1007/978-1-4939-7163-3_22. Link to article on publisher's siteDOI
10.1007/978-1-4939-7163-3_22Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43590PubMed ID
28752462Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1007/978-1-4939-7163-3_22