Title

Quantification of Z-AAT by a Z-Specific "Sandwich" ELISA

UMMS Affiliation

Horae Gene Therapy Center; Department of Pediatrics, Division of Pulmonary and Allergy

Publication Date

7-28-2017

Document Type

Book Chapter

Disciplines

Molecular Biology | Research Methods in Life Sciences

Abstract

This protocol describes an enzyme-linked immunosorbent assay (ELISA) to specifically detect Z-alpha-1 antitrypsin (AAT), the most common protein variant associated with alpha-1 antitrypsin deficiency. This "sandwich" ELISA relies on an anti-Z-AAT specific capture antibody and a HRP-conjugated anti-AAT detection antibody. This method would be of interest to identify and quantify Z-AAT in a variety of samples such as cell culture medium, cell or tissue lysate, animal or patient serum. Because this method is specific and sensitive, it would be particularly valuable for detection of Z-AAT in the presence of background M-AAT, for instance when quantifying silencing of Z-AAT in patients undergoing M-AAT augmentation therapy.

Keywords

AAT, Alpha-1 antitrypsin, Enzyme-linked immunosorbent assay, Sandwich ELISA, UMMS-PiZ-mAb, Z-AAT

DOI of Published Version

10.1007/978-1-4939-7163-3_22

Source

Methods Mol Biol. 2017;1639:223-226. doi: 10.1007/978-1-4939-7163-3_22. Link to article on publisher's site

Journal/Book/Conference Title

Methods in molecular biology (Clifton, N.J.)

Related Resources

Link to Article in PubMed

PubMed ID

28752462

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