Title

Influence of EBV on the peripheral blood memory B cell compartment

UMMS Affiliation

Department of Pediatrics; Program in Molecular Medicine

Publication Date

9-1-2007

Document Type

Article

Subjects

Antigens, CD27; B-Lymphocytes; Epstein-Barr Virus Infections; Gene Expression; *Genes, Immunoglobulin; Herpesvirus 4, Human; Humans; Immunoglobulin D; Immunoglobulin M; *Immunologic Memory; Molecular Sequence Data; Mutagenesis; Mutation; *Virus Latency

Disciplines

Immunology and Infectious Disease | Pediatrics

Abstract

Peripheral blood memory B cells latently infected with EBV bear somatic mutations and are typically isotype switched consistent with being classical Ag-selected memory B cells. In this work, we performed a comparative analysis of the expressed Ig genes between large sets of EBV-infected and uninfected peripheral blood B cells, isolated from the same infectious mononucleosis patients, to determine whether differences exist that could reveal the influence of EBV on the production and maintenance of these cells. We observed that EBV(+) cells on average accumulated more somatic hypermutations than EBV(-) cells. In addition, they had more replacement mutations and a higher replacement-silent ratio of mutations in their CDRs. We also found that EBV occupies a skewed niche within the memory compartment, due to its exclusion from the CD27(+)IgD(+)IgM(+) subset, but this skewing does not affect the overall structure of the compartment. These results indicate that EBV impacts the mutation and selection process of infected cells but that once they enter memory they cannot be distinguished from uninfected cells by host homeostasis mechanisms.

Source

J Immunol. 2007 Sep 1;179(5):3153-60.

Journal/Book/Conference Title

Journal of immunology (Baltimore, Md. : 1950)

Related Resources

Link to Article in PubMed

PubMed ID

17709530

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