Title
p47(phox) PX domain of NADPH oxidase targets cell membrane via moesin-mediated association with the actin cytoskeleton
UMMS Affiliation
Department of Pediatrics
Publication Date
2004-07-01
Document Type
Article
Subjects
Actins; Animals; COS Cells; Cell Membrane; Cricetinae; Cytoskeleton; Humans; K562 Cells; Microfilament Proteins; NADPH Oxidase; Phospholipids; Phosphoproteins; Protein Transport
Disciplines
Hematology | Oncology | Pediatrics
Abstract
Activation of phagocytic NADPH oxidase requires association of its cytosolic subunits with the membrane-bound flavocytochrome. Extensive phosphorylation of the p47(phox) subunit of NADPH oxidase marks the initiation of this activation process. The p47(phox) subunit then translocates to the plasma membrane, bringing the p67(phox) subunit to cytochrome b558 to form the active NADPH oxidase complex. However, the detailed mechanism for targeting the p47(phox) subunit to the cell membrane during activation still remains unclear. Here, we show that the p47(phox) PX domain is responsible for translocating the p47(phox) subunit to the plasma membrane for subsequent activation of NADPH oxidase. We also demonstrate that translocation of the p47(phox) PX domain to the plasma membrane is not due to interactions with phospholipids but rather to association with the actin cytoskeleton. This association is mediated by direct interaction between the p47(phox) PX domain and moesin.
DOI of Published Version
10.1002/jcb.20084
Source
J Cell Biochem. 2004 Jul 1;92(4):795-809. doi: 10.1002/jcb.20084
Journal/Book/Conference Title
Journal of cellular biochemistry
Related Resources
PubMed ID
15211576
Repository Citation
Zhan Y, He D, Newburger PE, Zhou GW. (2004). p47(phox) PX domain of NADPH oxidase targets cell membrane via moesin-mediated association with the actin cytoskeleton. Hematology/Oncology. https://doi.org/10.1002/jcb.20084. Retrieved from https://escholarship.umassmed.edu/peds_hematology/66