Plasma glutathione peroxidase deficiency and platelet insensitivity to nitric oxide in children with familial stroke
Department of Pediatrics
Adolescent; Adult; Blood Platelets; Cerebrovascular Disorders; Child; Family Health; Female; Glutathione Peroxidase; Humans; Male; Nitric Oxide; Pedigree
Hematology | Oncology | Pediatrics
In a previous report by Freedman et al (J Clin Invest. 1996;97:979-987), plasma from 2 brothers with stroke or transient ischemic attack inactivated the antiplatelet effects of nitric oxide (NO), and this effect was found to be a consequence of a deficiency of plasma glutathione peroxidase (GSH-Px). In this study, we attempted to define the generalizability of this deficiency by studying NO-mediated antiplatelet effects in 7 families with familial childhood stroke. Seven families with familial childhood stroke that consecutively presented to a large referral center were included in the study. We monitored ADP-induced aggregation of normal gel-filtered platelets (GFP) in platelet-poor plasma (PPP) from normal individuals and from patients in the presence or absence of an NO donor (S-nitroso-glutathione). Surface P-selectin expression of normal GFP in patients' PPP was analyzed by flow cytometry after incubation with a P-selectin-specific monoclonal antibody in the presence or absence of the NO donor. We also measured GSH-Px activity in plasmas from family members and normal controls using standard methods. In 6 of 7 families, NO failed to inhibit platelet P-selectin expression and platelet aggregation in PPP from the affected family members and some of their relatives. Of 4 families studied, 3 probands and their corresponding affected parent had 50% decrease in plasma GSH-Px activity. In some patients with childhood stroke, impaired metabolism of reactive oxygen species as a result of reduced GSH-Px activity results in NO insufficiency that affects normal platelet inhibitory mechanisms and predisposes to arterial thrombosis.
DOI of Published Version
Arterioscler Thromb Vasc Biol. 1999 Aug;19(8):2017-23. doi: 10.1161/01.ATV.19.8.2017
Arteriosclerosis, thrombosis, and vascular biology
Kenet, Gili; Freedman, Jane; Shenkman, Boris; Regina, Eskaraev; Brok-Simoni, Frida; Holzman, Fanny; Vavva; Brand, Nathan; Michelson, Alan D.; Trolliet, Maria; Loscalzo, Joseph; and Inbal, Aida, "Plasma glutathione peroxidase deficiency and platelet insensitivity to nitric oxide in children with familial stroke" (1999). Hematology/Oncology. 20.