Title
Cellular calibrators to quantitate T-cell receptor excision circles (TRECs) in clinical samples
UMMS Affiliation
New England Newborn Screening Program; Department of Pediatrics
Publication Date
11-1-2012
Document Type
Article
Subjects
Genes, T-Cell Receptor; Calibration; Neonatal Screening
Disciplines
Genetics and Genomics | Medical Genetics | Pediatrics
Abstract
T-cell receptor excision circles (TRECs) are circular DNA molecules formed during rearrangement of the T-cell receptor (TCR) genes during lymphocyte development. Copy number of the junctional portion of the δRec-ψJα TREC, assessed by quantitative PCR (qPCR) using DNA from dried blood spots (DBS), is a biomarker for newly formed T cells and absent or low numbers of TRECs indicate SCID (severe combined immunodeficiency) or T lymphocytopenia. No quantitation standard for TRECs exists. To permit comparison of TREC qPCR results with a reliable method for counting TRECs across different laboratories, we sought to construct a stable cell line containing a normal human chromosomal constitution and a single copy of the TREC junction sequence. A human EBV (Epstein Barr virus)-transformed B-cell line was transduced with a lentivirus encoding mCherry fluorescence, puromycin resistance and the δRec-ψJα TREC sequence. A TREC-EBV cell line, with each cell carrying a single lentiviral insertion was established, expanded and shown to have one TREC copy per diploid genome. Graded numbers of TREC-EBV cells added to aliquots of T lymphocyte depleted blood showed TREC copy number proportional to TREC-EBV cell number. TREC-EBV cells, therefore, constitute a reproducible cellular calibrator for TREC assays, useful for both population-based screening for severe combined immunodeficiency and evaluation of naïve T-cell production in clinical settings.
DOI of Published Version
10.1016/j.ymgme.2012.09.018
Source
Mol Genet Metab. 2012 Nov;107(3):586-91. doi: 10.1016/j.ymgme.2012.09.018.
Journal/Book/Conference Title
Molecular genetics and metabolism
Related Resources
PubMed ID
23062576
Repository Citation
Punwani, Divya; Gonzalez-Espinosa, Diana; Comeau, Anne Marie; Dutra, Amalia; Pak, Evgenia; and Puck, Jennifer, "Cellular calibrators to quantitate T-cell receptor excision circles (TRECs) in clinical samples" (2012). Genetics. 35.
https://escholarship.umassmed.edu/peds_genetics/35