Cytocompatible poly(ethylene glycol)-co-polycarbonate hydrogels cross-linked by copper-free, strain-promoted click chemistry

UMMS Affiliation

Department of Orthopedics and Physical Rehabilitation

Publication Date


Document Type



Alkynes; Animals; Azides; Biocompatible Materials; Bone Marrow Cells; Cell Survival; *Click Chemistry; Cyclization; Heterocyclic Compounds, 3-Ring; Hydrogels; Male; Molecular Structure; Polyethylene Glycols; Rats


Biochemistry, Biophysics, and Structural Biology | Cell and Developmental Biology | Medicine and Health Sciences


Strategies to encapsulate cells in cytocompatible three-dimensional hydrogels with tunable mechanical properties and degradability without harmful gelling conditions are highly desired for regenerative medicine applications. Here we reported a method for preparing poly(ethylene glycol)-co-polycarbonate hydrogels through copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. Hydrogels with varying mechanical properties were formed by "clicking" azido-functionalized poly(ethylene glycol)-co-polycarbonate macromers with dibenzocyclooctyne-functionalized poly(ethylene glycol) under physiological conditions within minutes. Bone marrow stromal cells encapsulated in these gels exhibited higher cellular viability than those encapsulated in photo-cross-linked poly(ethylene glycol) dimethacrylate. The precise control over the macromer compositions, cytocompatible SPAAC cross-linking, and the degradability of the polycarbonate segments make these hydrogels promising candidates for scaffold and stem cell assisted tissue repair and regeneration.

DOI of Published Version



Chem Asian J. 2011 Oct 4;6(10):2730-7. doi: 10.1002/asia.201100411. Epub 2011 Aug 24. Link to article on publisher's site

Journal/Book/Conference Title

Chemistry, an Asian journal

Related Resources

Link to Article in PubMed

PubMed ID