(alpha-NaYbF4:Tm(3+))/CaF2 core/shell nanoparticles with efficient near-infrared to near-infrared upconversion for high-contrast deep tissue bioimaging
Department of Orthopedics and Physical Rehabilitation
Animals; Calcium Fluoride; Contrast Media; Femur; Fluorides; Infrared Rays; Materials Testing; Mice; Microscopy, Fluorescence; Molecular Conformation; Muscle, Skeletal; Nanoparticles; Particle Size; Porosity; Rats; Swine; Yttrium
Biochemistry, Biophysics, and Structural Biology | Cell and Developmental Biology | Molecular, Cellular, and Tissue Engineering | Nanoscience and Nanotechnology
We describe the development of novel and biocompatible core/shell (alpha-NaYbF(4):Tm(3+))/CaF(2) nanoparticles that exhibit highly efficient NIR(in)-NIR(out) upconversion (UC) for high contrast and deep bioimaging. When excited at ~980 nm, these nanoparticles emit photoluminescence (PL) peaked at ~800 nm. The quantum yield of this UC PL under low power density excitation (~0.3 W/cm(2)) is 0.6 +/- 0.1%. This high UC PL efficiency is realized by suppressing surface quenching effects via heteroepitaxial growth of a biocompatible CaF(2) shell, which results in a 35-fold increase in the intensity of UC PL from the core. Small-animal whole-body UC PL imaging with exceptional contrast (signal-to-background ratio of 310) is shown using BALB/c mice intravenously injected with aqueously dispersed nanoparticles (700 pmol/kg). High-contrast UC PL imaging of deep tissues is also demonstrated, using a nanoparticle-loaded synthetic fibrous mesh wrapped around rat femoral bone and a cuvette with nanoparticle aqueous dispersion covered with a 3.2 cm thick animal tissue (pork).
DOI of Published Version
ACS Nano. 2012 Sep 25;6(9):8280-7. Epub 2012 Sep 4. Link to article on publisher's site
Chen G, Shen J, Ohulchansky TY, Patel NJ, Kutikov A, Li Z, Song J, Pandey RK, Agren H, Prasad PN, Han G. (2012). (alpha-NaYbF4:Tm(3+))/CaF2 core/shell nanoparticles with efficient near-infrared to near-infrared upconversion for high-contrast deep tissue bioimaging. Orthopedics and Physical Rehabilitation Publications. https://doi.org/10.1021/nn302972r. Retrieved from https://escholarship.umassmed.edu/ortho_pp/171