Amphiphilic degradable polymers for immobilization and sustained delivery of sphingosine 1-phosphate

UMMS Affiliation

Department of Orthopedics and Physical Rehabilitation

Publication Date


Document Type



Biochemistry, Biophysics, and Structural Biology


Controlled delivery of the angiogenic factor sphingosine 1-phosphate (S1P) represents a promising strategy for promoting vascularization during tissue repair and regeneration. In this study, we developed an amphiphilic biodegradable polymer platform for the stable encapsulation and sustained release of S1P. Mimicking the interaction between amphiphilic S1P and its binding proteins, a series of polymers with hydrophilic poly(ethylene glycol) core and lipophilic flanking segments of polylactide and/or poly(alkylated lactide) with different alkyl chain lengths were synthesized. These polymers were electrospun into fibrous meshes, and loaded with S1P in generally high loading efficiencies (>90%). Sustained S1P release from these scaffolds could be tuned by adjusting the alkyl chain length, blockiness and lipophilic block length, achieving 35-55% and 45-80% accumulative releases in the first 8h and by 7 days, respectively. Furthermore, using endothelial cell tube formation assay and chicken chorioallantoic membrane assay, we showed that the different S1P loading doses and release kinetics translated into distinct pro-angiogenic outcomes. These results suggest that these amphiphilic polymers are effective delivery vehicles for S1P and may be explored as tissue engineering scaffolds where the delivery of lipophilic or amphiphilic bioactive factors is desired. reserved.


Angiogenesis, Drug delivery, Amphiphilic copolymer, Sphingosine-1-phosphate, Tissue engineering

DOI of Published Version



Acta Biomater. 2014 Jul;10(7):3079-90. doi: 10.1016/j.actbio.2014.02.051. Epub 2014 Mar 12. Link to article on publisher's site

Journal/Book/Conference Title

Acta biomaterialia

Related Resources

Link to Article in PubMed

PubMed ID