Odgren Lab Publications
Title
CSF-1 regulation of osteoclastogenesis for tooth eruption
UMMS Affiliation
Department of Cell Biology
Publication Date
2005-08-20
Document Type
Article
Subjects
Animals; Dental Sac; Down-Regulation; Gene Expression Regulation, Developmental; Glycoproteins; Immunoenzyme Techniques; Macrophage Colony-Stimulating Factor; Osteoclasts; Osteoprotegerin; RNA, Small Interfering; Rats; Rats, Mutant Strains; Receptors, Cytoplasmic and Nuclear; Receptors, Tumor Necrosis Factor; Reverse Transcriptase Polymerase Chain Reaction; Tooth Eruption; Transfection
Disciplines
Cell Biology
Abstract
The dental follicle regulates the alveolar bone resorption needed for tooth eruption. In the rat first mandibular molar, a decrease in the expression of osteoprotegerin (OPG) in the dental follicle at day 3 enables the osteoclastogenesis needed for eruption to occur. Because colony-stimulating factor-1 (CSF-1) is maximally expressed in the dental follicle at day 3, it was hypothesized that CSF-1 down-regulates OPG gene expression in the dental follicle in vivo. To test this, we compared the expression of OPG in osteopetrotic toothless (tl/tl) rats deficient in CSF-1 with expression in their normal littermates for given ages. OPG gene expression was found to be higher in the dental follicle of the tl/tl mutants than in normals. Transfecting short interfering RNA specific for CSF-1 mRNA into dental follicle cells resulted in an up-regulation of OPG expression. Thus, these studies support our hypothesis that the down-regulation of OPG needed for tooth eruption is mediated by CSF-1.
Source
J Dent Res. 2005 Sep;84(9):837-41.
Journal/Book/Conference Title
Journal of dental research
Related Resources
PubMed ID
16109994
Repository Citation
Wise GE, Yao S, Odgren PR, Pan F. (2005). CSF-1 regulation of osteoclastogenesis for tooth eruption. Odgren Lab Publications. Retrieved from https://escholarship.umassmed.edu/odgren/12