UMMS Affiliation

MassBiologics; Department of Obstetrics and Gynecology; Department of Pediatrics, Division of Immunology and Infectious Disease; Department of Biochemistry and Molecular Pharmacology

Publication Date


Document Type



Female Urogenital Diseases and Pregnancy Complications | Maternal and Child Health | Obstetrics and Gynecology | Women's Health


Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.


diagnostic, isoforms, monoclonal antibody (mAb), preeclampsia, soluble fms-like tyrosine kinase 1 (sFlt1), splice variants, UMCCTS funding

DOI of Published Version



Int J Mol Sci. 2015 Jun 2;16(6):12436-53. doi: 10.3390/ijms160612436. Link to article on publisher's site

Journal/Book/Conference Title

International journal of molecular sciences


This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Link to Article in PubMed

PubMed ID


Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.