A role for the DnaJ homologue Scj1p in protein folding in the yeast endoplasmic reticulum
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDepartment of Biochemistry and Molecular Biology
Document Type
Journal ArticlePublication Date
1998-11-17Keywords
AllelesChaperonins
Endoplasmic Reticulum
Fungal Proteins
Gene Expression Regulation, Fungal
Glycosylation
HSP40 Heat-Shock Proteins
HSP70 Heat-Shock Proteins
Heat-Shock Proteins
Membrane Proteins
Mutagenesis
Oligosaccharides
Oxidative Stress
Protein Folding
RNA, Messenger
*Saccharomyces cerevisiae Proteins
Temperature
Yeasts
Biochemistry
Cell Biology
Molecular Biology
Pharmacology
Metadata
Show full item recordAbstract
Members of the eukaryotic heat shock protein 70 family (Hsp70s) are regulated by protein cofactors that contain domains homologous to bacterial DnaJ. Of the three DnaJ homologues in the yeast rough endoplasmic reticulum (RER; Scj1p, Sec63p, and Jem1p), Scj1p is most closely related to DnaJ, hence it is a probable cofactor for Kar2p, the major Hsp70 in the yeast RER. However, the physiological role of Scj1p has remained obscure due to the lack of an obvious defect in Kar2p-mediated pathways in scj1 null mutants. Here, we show that the Deltascj1 mutant is hypersensitive to tunicamycin or mutations that reduce N-linked glycosylation of proteins. Although maturation of glycosylated carboxypeptidase Y occurs with wild-type kinetics in Deltascj1 cells, the transport rate for an unglycosylated mutant carboxypeptidase Y (CPY) is markedly reduced. Loss of Scj1p induces the unfolded protein response pathway, and results in a cell wall defect when combined with an oligosaccharyltransferase mutation. The combined loss of both Scj1p and Jem1p exaggerates the sensitivity to hypoglycosylation stress, leads to further induction of the unfolded protein response pathway, and drastically delays maturation of an unglycosylated reporter protein in the RER. We propose that the major role for Scj1p is to cooperate with Kar2p to mediate maturation of proteins in the RER lumen.Source
J Cell Biol. 1998 Nov 16;143(4):921-33.Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42606PubMed ID
9817751Related Resources
Link to Article in PubMedCollections
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