The protein-tyrosine kinase fer associates with signaling complexes containing insulin receptor substrate-1 and phosphatidylinositol 3-kinase
Program in Molecular Medicine and Department of Biochemistry and Pharmacology
1-Phosphatidylinositol 3-Kinase; 3T3 Cells; Adipocytes; Amino Acid Sequence; Animals; COS Cells; Cell Differentiation; Cells, Cultured; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Gene Library; Immunoblotting; Insulin; Mice; Molecular Sequence Data; Phosphoproteins; Phosphorylation; Platelet-Derived Growth Factor; Precipitin Tests; Protein Binding; Protein Isoforms; Protein Structure, Tertiary; Protein-Tyrosine Kinases; Proto-Oncogene Proteins; Recombinant Fusion Proteins; Sequence Homology, Amino Acid; Signal Transduction; Time Factors; Transfection; Tyrosine; src Homology Domains
Life Sciences | Medicine and Health Sciences
In a screen for 3T3-F442A adipocyte proteins that bind SH2 domains, we isolated a cDNA encoding Fer, a nonreceptor protein-tyrosine kinase of the Fes/Fps family that contains a functional SH2 domain. A truncated splicing variant, iFer, was also cloned. iFer is devoid of both the tyrosine kinase domain and a functional SH2 domain but displays a unique 42-residue C terminus and retains the ability to form oligomers with Fer. Expression of both Fer and iFer proteins are strikingly increased upon differentiation of 3T3-L1 fibroblasts to adipocytes. Platelet-derived growth factor treatment of the cultured adipocytes caused rapid tyrosine phosphorylation of Fer and its recruitment to complexes containing platelet-derived growth factor receptor and the p85 regulatory subunit of phosphatidylinositol (PI) 3-kinase. Insulin treatment of 3T3-L1 adipocytes stimulated association of Fer with complexes containing tyrosine phosphorylated IRS-1 and PI 3-kinase but did not stimulate tyrosine phosphorylation of Fer. PI 3-kinase activity in anti-Fer immunoprecipitates was also acutely activated by insulin treatment of cultured adipocytes. These data demonstrate the presence of Fer tyrosine kinase in insulin signaling complexes, suggesting a role of Fer in insulin action.
DOI of Published Version
J Biol Chem. 2000 Dec 15;275(50):38995-9000. Link to article on publisher's site
The Journal of biological chemistry
Iwanishi, Masanori; Czech, Michael P.; and Cherniack, Andrew D., "The protein-tyrosine kinase fer associates with signaling complexes containing insulin receptor substrate-1 and phosphatidylinositol 3-kinase" (2000). Open Access Articles. 749.