Identification of a conserved ankyrin-binding motif in the family of sodium channel alpha subunits
Department of Cell Biology and Program in Neuroscience
Amino Acid Motifs; Amino Acid Sequence; Animals; Ankyrins; Cell Adhesion Molecules; Cell Line; Cell Membrane; Cells, Cultured; Cricetinae; DNA, Complementary; Hippocampus; Microscopy, Fluorescence; Molecular Sequence Data; Nerve Growth Factors; Neurons; Plasmids; Protein Binding; Protein Structure, Tertiary; Rats; Rats, Wistar; Recombinant Fusion Proteins; Sequence Homology, Amino Acid; Sodium Channels; Transfection; Two-Hybrid System Techniques
Life Sciences | Medicine and Health Sciences
Interactions with ankyrinG are crucial to the localization of voltage-gated sodium channels (VGSCs) at the axon initial segment and for neurons to initiate action potentials. However, the molecular nature of these interactions remains unclear. Here we report that VGSC-alpha, but not -beta, subunits bind to ankyrinG using pull-down assays. Further dissection of this activity identifies a conserved 9-amino acid motif ((V/A)P(I/L)AXXE(S/D)D) required for ankyrinG binding. This motif is also required for the localization of chimeric neurofascin/sodium channel molecules to the initial segment of cultured hippocampal neurons. The conserved nature of this motif suggests that it functions to localize sodium channels to a variety of "excitable" membrane domains both inside and outside of the nervous system.
DOI of Published Version
J Biol Chem. 2003 Jul 25;278(30):27333-9. Epub 2003 Apr 25. Link to article on publisher's site
The Journal of biological chemistry
Lemaillet, Guy; Walker, Barbara J.; and Lambert, Stephen, "Identification of a conserved ankyrin-binding motif in the family of sodium channel alpha subunits" (2003). Open Access Articles. 718.