Altered induction of the adaptive response to alkylation damage in Escherichia coli recF mutants

UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date


Document Type



Alkylation; Bacterial Proteins; Escherichia coli; Genes; *Genes, Bacterial; Kinetics; Methyl Methanesulfonate; Methylnitronitrosoguanidine; *Mutation; Operon; beta-Galactosidase


Life Sciences | Medicine and Health Sciences


Escherichia coli recF mutants are hypermutable when treated with methyl methanesulfonate (G. C. Walker, Mol. Gen. Genet. 152:93-103, 1977). In this study, methylation hypermutability of recF mutant strains was examined, and it was found that recF+ is required for normal induction of the adaptive response to alkylation damage. Although this regulatory effect of recF mutations results in reduced levels of enzymes that specifically repair methyl lesions in DNA, it only partially explains the hypermutability. Further examination showed that methylation hypermutability of recF mutant strains required a functional umuDC operon, a component of the SOS response. These results lead to the hypothesis that methylation hypermutability results from the effects of recF mutations on the induction of both the SOS response and the adaptive response.


J Bacteriol. 1989 Jan;171(1):99-103.

Journal/Book/Conference Title

Journal of bacteriology

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