Altered induction of the adaptive response to alkylation damage in Escherichia coli recF mutants
Department of Molecular Genetics and Microbiology
Alkylation; Bacterial Proteins; Escherichia coli; Genes; *Genes, Bacterial; Kinetics; Methyl Methanesulfonate; Methylnitronitrosoguanidine; *Mutation; Operon; beta-Galactosidase
Life Sciences | Medicine and Health Sciences
Escherichia coli recF mutants are hypermutable when treated with methyl methanesulfonate (G. C. Walker, Mol. Gen. Genet. 152:93-103, 1977). In this study, methylation hypermutability of recF mutant strains was examined, and it was found that recF+ is required for normal induction of the adaptive response to alkylation damage. Although this regulatory effect of recF mutations results in reduced levels of enzymes that specifically repair methyl lesions in DNA, it only partially explains the hypermutability. Further examination showed that methylation hypermutability of recF mutant strains required a functional umuDC operon, a component of the SOS response. These results lead to the hypothesis that methylation hypermutability results from the effects of recF mutations on the induction of both the SOS response and the adaptive response.
J Bacteriol. 1989 Jan;171(1):99-103.
Journal of bacteriology
Volkert, Michael R., "Altered induction of the adaptive response to alkylation damage in Escherichia coli recF mutants" (1989). Open Access Articles. 670.