Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli

UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date


Document Type



Bacteriophage lambda; Calcium; Chromosomes, Bacterial; Cloning, Molecular; DNA Primers; DNA, Superhelical; Escherichia coli; Gene Targeting; Genotype; Operon; Plasmids; Polymerase Chain Reaction; Recombinant Fusion Proteins; *Recombination, Genetic; Restriction Mapping


Life Sciences | Medicine and Health Sciences


Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.


J Bacteriol. 1998 Apr;180(8):2063-71.

Journal/Book/Conference Title

Journal of bacteriology

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Link to Article in PubMed

PubMed ID