Inhibition of apoptosis by ATFx: a novel role for a member of the ATF/CREB family of mammalian bZIP transcription factors
Howard Hughes Medical Institute and Programs in Molecular Medicine and Gene Function and Expression
Activating Transcription Factors; Acute-Phase Proteins; Animals; *Apoptosis; Carrier Proteins; Cell Cycle; Cell Division; Cell Line; Cell Survival; Cyclic AMP Response Element-Binding Protein; *DNA-Binding Proteins; Down-Regulation; Hela Cells; Humans; Interleukin-3; Leucine Zippers; Lipocalins; Mice; Oncogene Proteins; Proto-Oncogene Proteins; Recombinant Fusion Proteins; Transcription Factors
Life Sciences | Medicine and Health Sciences
The mammalian ATF/CREB family of transcription factors comprises a large group of basic-region leucine zipper (bZIP) proteins whose members mediate diverse transcriptional regulatory functions. Here we report that expression of a specific mouse ATF gene, ATFx, is down-regulated in a variety of cells undergoing apoptosis following growth factor deprivation. When stably expressed in an interleukin 3 (IL-3)-dependent cell line, ATFx suppresses apoptosis resulting from cytokine deprivation. Conversely, a dominant-negative ATFx mutant induces apoptosis of cells cultured in the presence of growth factors. We also show that 24p3, a secreted lipocalin that induces apoptosis when added to hematopoietic cells, represses ATFx expression. However, constitutive expression of ATFx renders cells resistant to 24p3-mediated apoptosis. Collectively, our results indicate that ATFx is an anti-apoptotic factor, a novel role for an ATF protein.
DOI of Published Version
Genes Dev. 2002 Jul 15;16(14):1806-14. Link to article on publisher's site
Genes and development
Persengiev, Stephan P.; Devireddy, Laxminarayana R.; and Green, Michael R., "Inhibition of apoptosis by ATFx: a novel role for a member of the ATF/CREB family of mammalian bZIP transcription factors" (2002). Open Access Articles. 586.