Identification of phosphatases for Smad in the BMP/DPP pathway
Program in Molecular Medicine; Department of Cancer Biology
Animals; Base Sequence; Bone Morphogenetic Proteins; DNA Primers; Drosophila; Drosophila Proteins; Phosphorylation; Polymerase Chain Reaction; Pyruvate Dehydrogenase (Lipoamide)-Phosphatase; Signal Transduction; Smad Proteins; Subcellular Fractions
Life Sciences | Medicine and Health Sciences
Phosphorylation of the SSXS motif of Smads is critical in activating the transforming growth factor beta (TGF-beta) and bone morphogenetic protein (BMP) pathways. However, the phosphatase(s) involved in dephosphorylating and hence inactivating Smads remained elusive. Through RNA interference (RNAi)-based screening of serine/threonine phosphatases in Drosophila S2 cells, we identified pyruvate dehydrogenase phosphatase (PDP) to be required for dephosphorylation of Mothers against Decapentaplegic (MAD), a Drosophila Smad. Biochemical and genetic evidence suggest that PDP directly dephosphorylates MAD and inhibits signal transduction of Decapentaplegic (DPP). We show that the mammalian PDPs are important in dephosphorylation of BMP-activated Smad1 but not TGF-beta-activated Smad2 or Smad3. Thus, PDPs specifically inactivate Smads in the BMP/DPP pathway.
DOI of Published Version
Genes Dev. 2006 Mar 15;20(6):648-53. Epub 2006 Mar 1. Link to article on publisher's site
Genes and development
Chen, Hong B.; Shen, Jiali; Ip, Y. Tony; and Xu, Lan, "Identification of phosphatases for Smad in the BMP/DPP pathway" (2006). Open Access Articles. 573.