Second-site reversion of a structural defect in bacteriophage T4 lysozyme
Department of Molecular Genetics and Microbiology
Bacteriophage T4; Bacteriophage lambda; Base Sequence; Genetic Vectors; Models, Molecular; Molecular Sequence Data; Muramidase; Mutagenesis; Plasmids; Polymerase Chain Reaction; *Protein Folding; Selection (Genetics); Sequence Analysis, DNA
Life Sciences | Medicine and Health Sciences
A plasmid-borne gene encoding bacteriophage T4 lysozyme with a structural mutation, Tyr161-Ala, was mutagenized by by the use of polymerase chain reaction. The mutagenized gene was inserted into a specialized bacteriophage lambda cloning vector that must acquire a functional lysozyme gene in order to form plaques. Functional variants of the mutant lysozyme were selected. Three compensatory second-site revertants were obtained: Thr152-Met, Lys43-Ile, and Thr151-Ala. The effects of these mutations are interpreted in light of previous structural and genetic studies of T4 lysozyme.
DOI of Published Version
FASEB J. 1996 Jan;10(1):159-63.
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Bouvier SE, Poteete AR. (1996). Second-site reversion of a structural defect in bacteriophage T4 lysozyme. Open Access Publications by UMMS Authors. https://doi.org/10.1096/fasebj.10.1.8566537. Retrieved from https://escholarship.umassmed.edu/oapubs/568