A composite tissue-engineered trachea using sheep nasal chondrocyte and epithelial cells

UMMS Affiliation

Center for Tissue Engineering

Publication Date


Document Type



Animals; Cell Count; Cell Culture Techniques; Cells, Cultured; Chondrocytes; Epithelial Cells; Glycosaminoglycans; Hydroxyproline; Microscopy, Phase-Contrast; Nose; Proteoglycans; Sheep; Tissue Engineering; Trachea


Life Sciences | Medicine and Health Sciences


This study evaluates the feasibility of producing a composite engineered tracheal equivalent composed of cylindrical cartilaginous structures with lumens lined with nasal epithelial cells. Chondrocytes and epithelial cells isolated from sheep nasal septum were cultured in Ham's F12 media. After 2 wk, chondrocyte suspensions were seeded onto a matrix of polyglycolic acid. Cell-polymer constructs were wrapped around silicon tubes and cultured in vitro for 1 wk, followed by implanting into subcutaneous pockets on the backs of nude mice. After 6 wk, epithelial cells were suspended in a hydrogel and injected into the embedded cartilaginous cylinders following removal of the silicon tube. Implants were harvested 4 wk later and analyzed. The morphology of implants resembles that of native sheep trachea. HandE staining shows the presence of mature cartilage and formation of a pseudo-stratified columnar epithelium, with a distinct interface between tissue-engineered cartilage and epithelium. Safranin-O staining shows that tissue-engineered cartilage is organized into lobules with round, angular lacunae, each containing a single chondrocyte. Proteoglycan and hydroxyproline contents are similar to native cartilage. This study demonstrates the feasibility of recreating the cartilage and epithelial portion of the trachea using tissue harvested in a single procedure. This has the potential to facilitate an autologous repair of segmental tracheal defects.

DOI of Published Version



FASEB J. 2003 May;17(8):823-8. Link to article on publisher's site

Journal/Book/Conference Title

The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Related Resources

Link to Article in PubMed

PubMed ID