Microinjection for precision genome editing in Caenorhabditis elegans
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Journal ArticlePublication Date
2021-09-01Keywords
CRISPRgenetics
model organisms
molecular biology
Amino Acids, Peptides, and Proteins
Computational Biology
Genomics
Molecular Biology
Nucleic Acids, Nucleotides, and Nucleosides
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In Caenorhabditis elegans, targeted genome editing techniques are now routinely used to generate germline edits. The remarkable ease of C. elegans germline editing is attributed to the syncytial nature of the pachytene ovary which is easily accessed by microinjection. This protocol describes the step-by-step details and troubleshooting tips for the entire CRISPR-Cas genome editing procedure, including gRNA design and microinjection of ribonucleoprotein complexes, followed by screening and genotyping in C. elegans, to help accessing this powerful genetic animal system. For complete details on the use and execution of this protocol, please refer to Ghanta and Mello (2020).Source
Ghanta KS, Ishidate T, Mello CC. Microinjection for precision genome editing in Caenorhabditis elegans. STAR Protoc. 2021 Sep 1;2(3):100748. doi: 10.1016/j.xpro.2021.100748. PMID: 34505086; PMCID: PMC8417391. Link to article on publisher's site
DOI
10.1016/j.xpro.2021.100748Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42734PubMed ID
34505086Related Resources
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Copyright 2021. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1016/j.xpro.2021.100748
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Except where otherwise noted, this item's license is described as Copyright 2021. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).