C. elegans LIN-28 controls temporal cell fate progression by regulating LIN-46 expression via the 5' UTR of lin-46 mRNA
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UMass Chan Affiliations
Program in Molecular MedicineDocument Type
Journal ArticlePublication Date
2021-09-07Keywords
5′ UTRLIN-28
LIN-46
Lin28
cell fate progression
cell proliferation
heterochronic
let-7
mRNA
pluripotency
Amino Acids, Peptides, and Proteins
Cell Biology
Developmental Biology
Nucleic Acids, Nucleotides, and Nucleosides
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Show full item recordAbstract
Lin28/LIN-28 is a conserved RNA-binding protein that promotes proliferation and pluripotency and can be oncogenic in mammals. Mammalian Lin28 and C. elegans LIN-28 have been shown to inhibit biogenesis of the conserved cellular differentiation-promoting microRNA let-7 by directly binding to unprocessed let-7 transcripts. Lin28/LIN-28 also bind and regulate many mRNAs in diverse cell types. However, the determinants and consequences of LIN-28-mRNA interactions are not well understood. Here, we report that C. elegans LIN-28 represses the expression of LIN-46, a downstream protein in the heterochronic pathway. We find that lin-28 and sequences within the lin-46 5' UTR are required to prevent LIN-46 expression at early larval stages. Moreover, we find that precocious LIN-46 expression caused by mutations in the lin-46 5' UTR is sufficient to cause precocious heterochronic defects similar to those of lin-28(lf) animals. Thus, our findings demonstrate the biological importance of the regulation of individual target mRNAs by LIN-28.Source
Ilbay O, Nelson C, Ambros V. C. elegans LIN-28 controls temporal cell fate progression by regulating LIN-46 expression via the 5' UTR of lin-46 mRNA. Cell Rep. 2021 Sep 7;36(10):109670. doi: 10.1016/j.celrep.2021.109670. PMID: 34496246; PMCID: PMC8445076. Link to article on publisher's site
DOI
10.1016/j.celrep.2021.109670Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42689PubMed ID
34496246Related Resources
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Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1016/j.celrep.2021.109670
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Except where otherwise noted, this item's license is described as Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).