Department of Medicine, Division of Infectious Diseases and Immunology; Graduate School of Biomedical Sciences
Bacterial Infections and Mycoses | Immunology and Infectious Disease | Microbiology
Exposure to the mold, Aspergillus, is ubiquitous and generally has no adverse consequences in immunocompetent persons. However, invasive and allergic aspergillosis can develop in immunocompromised and atopic individuals, respectively. Previously, we demonstrated that mouse lung eosinophils produce IL-17 in response to stimulation by live conidia and antigens of A. fumigatus. Here, we utilized murine models of allergic and acute pulmonary aspergillosis to determine the association of IL-23, IL-23R and RORgammat with eosinophil IL-17 expression. Following A. fumigatus stimulation, a population of lung eosinophils expressed RORgammat, the master transcription factor for IL-17 regulation. Eosinophil RORgammat expression was demonstrated by flow cytometry, confocal microscopy, western blotting and an mCherry reporter mouse. Both nuclear and cytoplasmic localization of RORgammat in eosinophils were observed, although the former predominated. A population of lung eosinophils also expressed IL-23R. While expression of IL-23R was positively correlated with expression of RORgammat, expression of RORgammat and IL-17 was similar when comparing lung eosinophils from A. fumigatus-challenged wild-type and IL-23p19-/- mice. Thus, in allergic and acute models of pulmonary aspergillosis, lung eosinophils express IL-17, RORgammat and IL-23R. However, IL-23 is dispensable for production of IL-17 and RORgammat.
pulmonary aspergillosis, lung eosinophils
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DOI of Published Version
Yadav B, Specht CA, Lee CK, Pokrovskii M, Huh JR, Littman DR, Levitz SM. Lung eosinophils elicited during allergic and acute aspergillosis express RORγt and IL-23R but do not require IL-23 for IL-17 production. PLoS Pathog. 2021 Aug 31;17(8):e1009891. doi: 10.1371/journal.ppat.1009891. PMID: 34464425; PMCID: PMC8437264. Link to article on publisher's site
Yadav B, Specht CA, Lee CK, Pokrovskii M, Huh JR, Littman DR, Levitz SM. (2021). Lung eosinophils elicited during allergic and acute aspergillosis express RORgammat and IL-23R but do not require IL-23 for IL-17 production. Open Access Publications by UMass Chan Authors. https://doi.org/10.1371/journal.ppat.1009891. Retrieved from https://escholarship.umassmed.edu/oapubs/4911
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