Title

Noncanonical cytoplasmic poly(A) polymerases regulate RNA levels, alternative RNA processing, and synaptic plasticity but not hippocampal-dependent behaviours

UMMS Affiliation

Program in Molecular Medicine

Publication Date

2021-07-01

Document Type

Article

Disciplines

Amino Acids, Peptides, and Proteins | Enzymes and Coenzymes | Neuroscience and Neurobiology | Nucleic Acids, Nucleotides, and Nucleosides

Abstract

Noncanonical poly(A) polymerases are frequently tethered to mRNA 3' untranslated regions and regulate poly(A) tail length and resulting translation. In the brain, one such poly(A) polymerase is Gld2, which is anchored to mRNA by the RNA-binding protein CPEB1 to control local translation at postsynaptic regions. Depletion of CPEB1 or Gld2 from the mouse hippocampus results in a deficit in long-term potentiation (LTP), but only depletion of CPEB1 alters animal behaviour. To test whether a related enzyme, Gld4, compensates for the lack of Gld2, we separately or simultaneously depleted both proteins from hippocampal area CA1 and again found little change in animal behaviour, but observed a deficit in LTP as well as an increase in long-term depression (LTD), two forms of protein synthesis-dependent synaptic plasticity. RNA-seq data from Gld2, Gld4, and Gld2/Gld4-depleted hippocampus show widespread changes in steady state RNA levels, alternative splicing, and alternative poly(A) site selection. Many of the RNAs subject to these alterations encode proteins that mediate synaptic function, suggesting a molecular foundation for impaired synaptic plasticity.

Keywords

Noncanonical poly(A) polymerase, RNA-seq, alternative RNA processing, animal behaviour, synaptic plasticity

DOI of Published Version

10.1080/15476286.2020.1824061

Source

Mansur F, Alarcon JM, Stackpole EE, Wang R, Richter JD. Noncanonical cytoplasmic poly(A) polymerases regulate RNA levels, alternative RNA processing, and synaptic plasticity but not hippocampal-dependent behaviours. RNA Biol. 2021 Jul;18(7):962-971. doi: 10.1080/15476286.2020.1824061. Epub 2020 Oct 12. PMID: 32954964; PMCID: PMC8216208. Link to article on publisher's site

Journal/Book/Conference Title

RNA biology

Related Resources

Link to Article in PubMed

PubMed ID

32954964

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