UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

2021-05-06

Document Type

Article

Disciplines

Cancer Biology | Cell Biology

Abstract

Herein, a set of optogenetic tools (designated LiPOP) that enable photoswitchable necroptosis and pyroptosis in live cells with varying kinetics, is introduced. The LiPOP tools allow reconstruction of the key molecular steps involved in these two non-apoptotic cell death pathways by harnessing the power of light. Further, the use of LiPOPs coupled with upconversion nanoparticles or bioluminescence is demonstrated to achieve wireless optogenetic or chemo-optogenetic killing of cancer cells in multiple mouse tumor models. LiPOPs can trigger necroptotic and pyroptotic cell death in cultured prokaryotic or eukaryotic cells and in living animals, and set the stage for studying the role of non-apoptotic cell death pathways during microbial infection and anti-tumor immunity.

Keywords

bioluminescence, necroptosis, optogenetics, pyroptosis, upconversion nanoparticles

Rights and Permissions

Copyright © 2021 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

DOI of Published Version

10.1002/advs.202100424

Source

He L, Huang Z, Huang K, Chen R, Nguyen NT, Wang R, Cai X, Huang Z, Siwko S, Walker JR, Han G, Zhou Y, Jing J. Optogenetic Control of Non-Apoptotic Cell Death. Adv Sci (Weinh). 2021 May 6;8(13):2100424. doi: 10.1002/advs.202100424. PMID: 34540558; PMCID: PMC8438606. Link to article on publisher's site

Journal/Book/Conference Title

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Related Resources

Link to Article in PubMed

PubMed ID

34540558

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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