Department of Biochemistry and Molecular Pharmacology
Cancer Biology | Cell Biology
Herein, a set of optogenetic tools (designated LiPOP) that enable photoswitchable necroptosis and pyroptosis in live cells with varying kinetics, is introduced. The LiPOP tools allow reconstruction of the key molecular steps involved in these two non-apoptotic cell death pathways by harnessing the power of light. Further, the use of LiPOPs coupled with upconversion nanoparticles or bioluminescence is demonstrated to achieve wireless optogenetic or chemo-optogenetic killing of cancer cells in multiple mouse tumor models. LiPOPs can trigger necroptotic and pyroptotic cell death in cultured prokaryotic or eukaryotic cells and in living animals, and set the stage for studying the role of non-apoptotic cell death pathways during microbial infection and anti-tumor immunity.
bioluminescence, necroptosis, optogenetics, pyroptosis, upconversion nanoparticles
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Copyright © 2021 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
DOI of Published Version
He L, Huang Z, Huang K, Chen R, Nguyen NT, Wang R, Cai X, Huang Z, Siwko S, Walker JR, Han G, Zhou Y, Jing J. Optogenetic Control of Non-Apoptotic Cell Death. Adv Sci (Weinh). 2021 May 6;8(13):2100424. doi: 10.1002/advs.202100424. PMID: 34540558; PMCID: PMC8438606. Link to article on publisher's site
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
He L, Huang Z, Huang K, Chen R, Nguyen NT, Wang R, Cai X, Huang Z, Siwko S, Walker JR, Han G, Zhou Y, Jing J. (2021). Optogenetic Control of Non-Apoptotic Cell Death. Open Access Publications by UMass Chan Authors. https://doi.org/10.1002/advs.202100424. Retrieved from https://escholarship.umassmed.edu/oapubs/4821
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This work is licensed under a Creative Commons Attribution 4.0 License.