UMMS Affiliation
Department of Microbiology and Physiological Systems
Publication Date
2021-05-17
Document Type
Article
Disciplines
Bacteria | Bacterial Infections and Mycoses | Hemic and Immune Systems | Immunology of Infectious Disease | Immunopathology | Infectious Disease
Abstract
T cell immunity is essential for the control of tuberculosis (TB), an important disease of the lung, and is generally studied in humans using peripheral blood cells. Mounting evidence, however, indicates that tissue-resident memory T cells (Trms) are superior at controlling many pathogens, including Mycobacterium tuberculosis (M. tuberculosis), and can be quite different from those in circulation. Using freshly resected lung tissue, from individuals with active or previous TB, we identified distinct CD4+ and CD8+ Trm-like clusters within TB-diseased lung tissue that were functional and enriched for IL-17-producing cells. M. tuberculosis-specific CD4+ T cells producing TNF-alpha, IL-2, and IL-17 were highly expanded in the lung compared with matched blood samples, in which IL-17+ cells were largely absent. Strikingly, the frequency of M. tuberculosis-specific lung T cells making IL-17, but not other cytokines, inversely correlated with the plasma IL-1beta levels, suggesting a potential link with disease severity. Using a human granuloma model, we showed the addition of either exogenous IL-17 or IL-2 enhanced immune control of M. tuberculosis and was associated with increased NO production. Taken together, these data support an important role for M. tuberculosis-specific Trm-like, IL-17-producing cells in the immune control of M. tuberculosis in the human lung.
Keywords
Bacterial infections, Immunology, Infectious disease, T cells
Rights and Permissions
© 2021 Ogongo et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
DOI of Published Version
10.1172/JCI142014
Source
Ogongo P, Tezera LB, Ardain A, Nhamoyebonde S, Ramsuran D, Singh A, Ng'oepe A, Karim F, Naidoo T, Khan K, Dullabh KJ, Fehlings M, Lee BH, Nardin A, Lindestam Arlehamn CS, Sette A, Behar SM, Steyn AJ, Madansein R, Kløverpris HN, Elkington PT, Leslie A. Tissue-resident-like CD4+ T cells secreting IL-17 control Mycobacterium tuberculosis in the human lung. J Clin Invest. 2021 May 17;131(10):e142014. doi: 10.1172/JCI142014. PMID: 33848273; PMCID: PMC8121523. Link to article on publisher's site
Journal/Book/Conference Title
The Journal of clinical investigation
Related Resources
PubMed ID
33848273
Repository Citation
Ogongo P, Tezera LB, Ardain A, Nhamoyebonde S, Ramsuran D, Singh A, Ng'oepe A, Karim F, Naidoo T, Khan K, Dullabh KJ, Fehlings M, Lee BH, Nardin A, Lindestam Arlehamn CS, Sette A, Behar SM, Steyn AJ, Madansein R, Kloverpris HN, Elkington PT, Leslie A. (2021). Tissue-resident-like CD4+ T cells secreting IL-17 control Mycobacterium tuberculosis in the human lung. Open Access Publications by UMMS Authors. https://doi.org/10.1172/JCI142014. Retrieved from https://escholarship.umassmed.edu/oapubs/4744
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Bacteria Commons, Bacterial Infections and Mycoses Commons, Hemic and Immune Systems Commons, Immunology of Infectious Disease Commons, Immunopathology Commons, Infectious Disease Commons