PIE-1 SUMOylation promotes germline fates and piRNA-dependent silencing in C. elegans
Authors
Kim, HeesunDing, Yue-He
Lu, Shan
Zuo, Mei-Qing
Tan, Wendy
Conte, Darryl Jr.
Dong, Meng-Qiu
Mello, Craig C.
Document Type
Journal ArticlePublication Date
2021-05-18Keywords
C. elegansNuRD complex
SUMO pathway
genetics
genomics
germline chromatin
germline fate
Amino Acids, Peptides, and Proteins
Genetics and Genomics
Metadata
Show full item recordAbstract
Germlines shape and balance heredity, integrating and regulating information from both parental and foreign sources. Insights into how germlines handle information have come from the study of factors that specify or maintain the germline fate. In early Caenorhabditis elegans embryos, the CCCH zinc finger protein PIE-1 localizes to the germline where it prevents somatic differentiation programs. Here, we show that PIE-1 also functions in the meiotic ovary where it becomes SUMOylated and engages the small ubiquitin-like modifier (SUMO)-conjugating machinery. Using whole-SUMO-proteome mass spectrometry, we identify HDAC SUMOylation as a target of PIE-1. Our analyses of genetic interactions between pie-1 and SUMO pathway mutants suggest that PIE-1 engages the SUMO machinery both to preserve the germline fate in the embryo and to promote Argonaute-mediated surveillance in the adult germline.Source
Kim H, Ding YH, Lu S, Zuo MQ, Tan W, Conte D Jr, Dong MQ, Mello CC. PIE-1 SUMOylation promotes germline fates and piRNA-dependent silencing in C. elegans. Elife. 2021 May 18;10:e63300. doi: 10.7554/eLife.63300. PMID: 34003111; PMCID: PMC8131105. Link to article on publisher's site
DOI
10.7554/eLife.63300Permanent Link to this Item
http://hdl.handle.net/20.500.14038/41917PubMed ID
34003111Related Resources
Rights
Copyright © 2021, Kim et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.7554/eLife.63300
Scopus Count
Except where otherwise noted, this item's license is described as Copyright © 2021, Kim et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.