UMMS Affiliation
Department of Molecular, Cell and Cancer Biology
Publication Date
2021-04-20
Document Type
Article
Disciplines
Cell Biology | Developmental Biology | Research Methods in Life Sciences
Abstract
This protocol describes the embedding and processing of Drosophila pupae in paraffin to monitor tissue changes during development. Although multiple methods are available to evaluate developmental changes in Drosophila embryos, imaging detailed changes during metamorphosis is challenging as the animal is enclosed in the cuticle, rendering it inaccessible to whole mount imaging. Here, we present a protocol that focuses on developmental clearance of the larval salivary glands in Drosophila pupae that can be extended to examine other tissues/stages for similar purposes. For complete details on the use and execution of this protocol, please refer to Velentzas et al. (2018).
Keywords
Cell Biology, Developmental biology, Microscopy, Model Organisms
Rights and Permissions
Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI of Published Version
10.1016/j.xpro.2021.100473
Source
Velentzas PD, Baehrecke EH. Histological assessment of developmental cell death in Drosophila pupae. STAR Protoc. 2021 Apr 20;2(2):100473. doi: 10.1016/j.xpro.2021.100473. PMID: 33997806; PMCID: PMC8091131. Link to article on publisher's site
Journal/Book/Conference Title
STAR protocols
Related Resources
PubMed ID
33997806
Repository Citation
Velentzas PD, Baehrecke EH. (2021). Histological assessment of developmental cell death in Drosophila pupae. Open Access Publications by UMMS Authors. https://doi.org/10.1016/j.xpro.2021.100473. Retrieved from https://escholarship.umassmed.edu/oapubs/4712
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Cell Biology Commons, Developmental Biology Commons, Research Methods in Life Sciences Commons