UMMS Affiliation

Department of Molecular, Cell and Cancer Biology

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Document Type



Cell Biology | Developmental Biology | Research Methods in Life Sciences


This protocol describes the embedding and processing of Drosophila pupae in paraffin to monitor tissue changes during development. Although multiple methods are available to evaluate developmental changes in Drosophila embryos, imaging detailed changes during metamorphosis is challenging as the animal is enclosed in the cuticle, rendering it inaccessible to whole mount imaging. Here, we present a protocol that focuses on developmental clearance of the larval salivary glands in Drosophila pupae that can be extended to examine other tissues/stages for similar purposes. For complete details on the use and execution of this protocol, please refer to Velentzas et al. (2018).


Cell Biology, Developmental biology, Microscopy, Model Organisms

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Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (

DOI of Published Version



Velentzas PD, Baehrecke EH. Histological assessment of developmental cell death in Drosophila pupae. STAR Protoc. 2021 Apr 20;2(2):100473. doi: 10.1016/j.xpro.2021.100473. PMID: 33997806; PMCID: PMC8091131. Link to article on publisher's site

Journal/Book/Conference Title

STAR protocols

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Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.