UMMS Affiliation

Division of Infectious Diseases and Immunology, Department of Medicine

Publication Date

2021-04-14

Document Type

Article

Disciplines

Immunoprophylaxis and Therapy | Virology | Virus Diseases

Abstract

INTRODUCTION: Poliovirus causes paralysis by infecting the nervous system. Currently, 2 types of polio vaccine are given in many countries in polio eradication program including inactivated polio vaccine (IPV) and oral polio vaccine (OPV). Because of OPV-related paralysis, OPV should be replaced by IPV.

METHODS: The aim of this study was to prepare the gamma-irradiated IPV and determine its effectiveness compared with the commercial vaccine (OPV) in the mouse model. The virus titration of OPV was determined and then inactivated by the appropriate dose of gamma radiation into an irradiated vaccine formula. The vaccine was inoculated in BALB/c mice in 2 different formulations of intramuscular injection with 2-week intervals. The level of anti-polio-neutralizing antibody and polio-specific splenocyte proliferation assay were evaluated by collecting the blood samples and spleens of the vaccinated groups with conventional vaccine and irradiated vaccine.

RESULTS: There was a significant increase in the neutralizing antibody titration between all of the vaccinated groups and negative control group (A) (p < 0.05). And it shows that the IPV by gamma irradiation has the highest antibody titration. Also, the increasing of stimulation index value in the B* group, F group, and G group was the most against other groups. Furthermore, the neutralizing anti-serum titer and splenic lymphocyte proliferation assay show humoral and cellular immunity were significantly increased in the irradiated vaccine group as compared with conventional group.

CONCLUSION: According to the results, gamma-irradiated IPV could induce humoral and cellular immunity in vaccinated mouse groups, so the irradiated poliovirus could be recommended as a good candidate vaccine to prevent the transport of poliovirus to the central nervous system and thus protect against paralysis.

Keywords

Adjuvant, Immunity, Poliovirus, Radiation, Vaccine

Rights and Permissions

© 2021 The Author(s). This article is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC). Usage and distribution for commercial purposes requires written permission.

DOI of Published Version

10.1159/000515392

Source

Mollaei Alamuti M, Ravanshad M, Motamedi-Sedeh F, Nabizadeh A, Ahmadi E, Hossieni SM. Immune Response of Gamma-Irradiated Inactivated Bivalent Polio Vaccine Prepared plus Trehalose as a Protein Stabilizer in a Mouse Model. Intervirology. 2021;64(3):140-146. doi: 10.1159/000515392. Epub 2021 Apr 14. PMID: 33853059. Link to article on publisher's site

Journal/Book/Conference Title

Intervirology

Related Resources

Link to Article in PubMed

PubMed ID

33853059

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

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