UMMS Affiliation

Program in Systems Biology; Graduate School of Biomedical Sciences

Publication Date

2021-02-03

Document Type

Article

Disciplines

Cancer Biology | Cell Biology | Systems Biology

Abstract

Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity. This is challenging, requiring time-lapse microscopy and genetically encoded labels to distinguish live and dead cells. Here, we describe fluorescence-based and lysis-dependent inference of cell death kinetics (FLICK). This method requires only a standard fluorescence plate reader, retaining the high-throughput nature and broad accessibility of common viability assays. However, FLICK specifically quantifies death, including an accurate inference of death kinetics. For complete details on the use and execution of this protocol, please refer to Richards et al. (2020).

Keywords

Cancer, Cell-based assays, High-throughput screening

Rights and Permissions

Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

DOI of Published Version

10.1016/j.xpro.2021.100327

Source

Richards R, Honeywell ME, Lee MJ. FLICK: an optimized plate reader-based assay to infer cell death kinetics. STAR Protoc. 2021 Feb 3;2(1):100327. doi: 10.1016/j.xpro.2021.100327. PMID: 33659903; PMCID: PMC7890003. Link to article on publisher's site

Journal/Book/Conference Title

STAR protocols

Related Resources

Link to Article in PubMed

PubMed ID

33659903

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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