Program in Systems Biology; Graduate School of Biomedical Sciences
Cancer Biology | Cell Biology | Systems Biology
Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity. This is challenging, requiring time-lapse microscopy and genetically encoded labels to distinguish live and dead cells. Here, we describe fluorescence-based and lysis-dependent inference of cell death kinetics (FLICK). This method requires only a standard fluorescence plate reader, retaining the high-throughput nature and broad accessibility of common viability assays. However, FLICK specifically quantifies death, including an accurate inference of death kinetics. For complete details on the use and execution of this protocol, please refer to Richards et al. (2020).
Cancer, Cell-based assays, High-throughput screening
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Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI of Published Version
Richards R, Honeywell ME, Lee MJ. FLICK: an optimized plate reader-based assay to infer cell death kinetics. STAR Protoc. 2021 Feb 3;2(1):100327. doi: 10.1016/j.xpro.2021.100327. PMID: 33659903; PMCID: PMC7890003. Link to article on publisher's site
Richards R, Honeywell ME, Lee MJ. (2021). FLICK: an optimized plate reader-based assay to infer cell death kinetics. Open Access Publications by UMMS Authors. https://doi.org/10.1016/j.xpro.2021.100327. Retrieved from https://escholarship.umassmed.edu/oapubs/4622
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This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.