Department of Molecular, Cell and Cancer Biology; MassBiologics
Amino Acids, Peptides, and Proteins | Cell Biology | Cellular and Molecular Physiology | Developmental Biology | Enzymes and Coenzymes | Genetics
The initiator caspase Dronc is the only CARD-domain containing caspase in Drosophila and is essential for apoptosis. Here, we report that homozygous dronc mutant adult animals are short-lived due to the presence of a poorly developed, defective and leaky intestine. Interestingly, this mutant phenotype can be significantly rescued by enteroblast-specific expression of dronc(+) in dronc mutant animals, suggesting that proper Dronc function specifically in enteroblasts, one of four cell types in the intestine, is critical for normal development of the intestine. Furthermore, enteroblast-specific knockdown of dronc in adult intestines triggers hyperplasia and differentiation defects. These enteroblast-specific functions of Dronc do not require the apoptotic pathway and thus occur in a non-apoptotic manner. In summary, we demonstrate that an apoptotic initiator caspase has a very critical non-apoptotic function for normal development and for the control of the cell lineage in the adult midgut and therefore for proper physiology and homeostasis.
Cell biology, Genetics
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DOI of Published Version
Lindblad JL, Tare M, Amcheslavsky A, Shields A, Bergmann A. Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine. Sci Rep. 2021 Jan 29;11(1):2645. doi: 10.1038/s41598-021-81261-0. PMID: 33514791; PMCID: PMC7846589. Link to article on publisher's site
Lindblad JL, Tare M, Amcheslavsky A, Shields A, Bergmann A. (2021). Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine. Open Access Publications by UMass Chan Authors. https://doi.org/10.1038/s41598-021-81261-0. Retrieved from https://escholarship.umassmed.edu/oapubs/4571
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This work is licensed under a Creative Commons Attribution 4.0 License.