Title
Fucoxanthin attenuates LPS-induced acute lung injury via inhibition of the TLR4/MYD88 signaling axis
UMMS Affiliation
Department of Molecular, Cell and Cancer Biology
Publication Date
2020-12-11
Document Type
Article
Disciplines
Cell Biology | Cellular and Molecular Physiology | Molecular Biology | Respiratory Tract Diseases
Abstract
Acute lung injury (ALI) is a critical clinical condition with a high mortality rate. It is believed that the inflammatory storm is a critical contributor to the occurrence of ALI. Fucoxanthin is a natural extract from marine seaweed with remarkable biological properties, including antioxidant, anti-tumor, and anti-obesity. However, the anti-inflammatory activity of Fucoxanthin has not been extensively studied. The current study aimed to elucidate the effects and the molecular mechanism of Fucoxanthin on lipopolysaccharide-induced acute lung injury. In this study, Fucoxanthin efficiently reduced the mRNA expression of pro-inflammatory factors, including IL-10, IL-6, iNOS, and Cox-2, and down-regulated the NF-kappaB signaling pathway in Raw264.7 macrophages. Furthermore, based on the network pharmacological analysis, our results showed that anti-inflammation signaling pathways were screened as fundamental action mechanisms of Fucoxanthin on ALI. Fucoxanthin also significantly ameliorated the inflammatory responses in LPS-induced ALI mice. Interestingly, our results revealed that Fucoxanthin prevented the expression of TLR4/MyD88 in Raw264.7 macrophages. We further validated Fucoxanthin binds to the TLR4 pocket using molecular docking simulations. Altogether, these results suggest that Fucoxanthin suppresses the TLR4/MyD88 signaling axis by targeting TLR4, which inhibits LPS-induced ALI, and fucoxanthin inhibition may provide a novel strategy for controlling the initiation and progression of ALI.
Keywords
acute lung injury, fucoxanthin, LPS, TLR4, MyD88, NF-κB
Rights and Permissions
Copyright: © 2020 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
10.18632/aging.202309
Source
Li X, Huang R, Liu K, Li M, Luo H, Cui L, Huang L, Luo L. Fucoxanthin attenuates LPS-induced acute lung injury via inhibition of the TLR4/MYD88 signaling axis. Aging (Albany NY). 2020 Dec 11;13(2):2655-2667. doi: 10.18632/aging.202309. Epub ahead of print. PMID: 33323555. Link to article on publisher's site
Journal/Book/Conference Title
Aging
Related Resources
PubMed ID
33323555
Repository Citation
Li X, Huang R, Liu K, Li M, Luo H, Cui L, Huang L, Luo L. (2020). Fucoxanthin attenuates LPS-induced acute lung injury via inhibition of the TLR4/MYD88 signaling axis. Open Access Publications by UMMS Authors. https://doi.org/10.18632/aging.202309. Retrieved from https://escholarship.umassmed.edu/oapubs/4485
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
Included in
Cell Biology Commons, Cellular and Molecular Physiology Commons, Molecular Biology Commons, Respiratory Tract Diseases Commons