UMMS Affiliation

Program in Molecular Medicine; Program in Systems Biology

Publication Date

2020-11-18

Document Type

Article

Disciplines

Cell Biology | Cellular and Molecular Physiology | Fungi | Molecular Biology | Systems Biology

Abstract

Dormancy is colloquially considered as extending lifespan by being still. Starved yeasts form dormant spores that wake-up (germinate) when nutrients reappear but cannot germinate (die) after some time. What sets their lifespans and how they age are open questions because what processes occur-and by how much-within each dormant spore remains unclear. With single-cell-level measurements, we discovered how dormant yeast spores age and die: spores have a quantifiable gene-expressing ability during dormancy that decreases over days to months until it vanishes, causing death. Specifically, each spore has a different probability of germinating that decreases because its ability to-without nutrients-express genes decreases, as revealed by a synthetic circuit that forces GFP expression during dormancy. Decreasing amounts of molecules required for gene expression-including RNA polymerases-decreases gene-expressing ability which then decreases chances of germinating. Spores gradually lose these molecules because they are produced too slowly compared with their degradations, causing gene-expressing ability to eventually vanish and, thus, death. Our work provides a systems-level view of dormancy-to-death transition.

Keywords

ageing, dormancy, gene expression, germination, yeast spores

Rights and Permissions

Copyright 2020 The Authors. Published under the terms of the CC BY 4.0 license.

DOI of Published Version

10.15252/msb.20199245

Source

Maire T, Allertz T, Betjes MA, Youk H. Dormancy-to-death transition in yeast spores occurs due to gradual loss of gene-expressing ability. Mol Syst Biol. 2020 Nov;16(11):e9245. doi: 10.15252/msb.20199245. PMID: 33206464; PMCID: PMC7673291. Link to article on publisher's site

Journal/Book/Conference Title

Molecular systems biology

Related Resources

Link to Article in PubMed

PubMed ID

33206464

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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